METFORMIN-SENSITIVE ION-SELECTIVE ELECTRODE

Metformin in its protonated form as metformin hydrochloride is used worldwide an advanced antidiabetic drug for type 2 diabetes. Hyperinsulinemia and insulin resistance of cells are the main causes this disease. For patients with diabetes, (MET) works by improving cell sensitivity to insulin.
 It has been shown that methyl orange (MO) forms ionic associate can be isolated solid suitable creation plasticized membrane potentiometric metformin-sensitive sensors.
 The energy efficiency IA formation substantiated method mathematical modeling. Molecular modeling MO- + MET+ systems related calculations were performed using PM3 various initial variants counter ion relative each other (single point procedure). Geometric optimization ions was molecular mechanics MM+. standard enthalpy (ΔH0) association “MET+ MO-” determined semi-empirical PM3. difference sum energies components 258 kJ/mol. Therefore, process thermodynamically advantageous.
 Modeling composition carried out. results study influence nature plasticizers on electroanalytical properties developed sensors indicate best plasticizer system TCP or DNF. these solvents, product dielectric constant Rohrschneider polarity (ε × PR) 123.5 175, respectively. less effective (DBF DEF), values 235.6 326 membranes same content one homologous series (DEF, DBF, DOF, DNF), slope Nernst function decreases increasing solvent. working pH range electrode from 11. drift potential does not exceed 1-3 mV/day. Stable potentials set 5-15 s. stability characteristics optimized traced at least three months. show satisfactory selectivity relation a number substances ions. 300-1000 amount glucose, starch, polyvinyl alcohol, Na+, K+, Mg2+, Ca2+, Ba2+ do interfere MET determination. This, turn, allows practical use MET-sensitive variety objects.
 A determination developed, which tested dosage forms.